Composite

Part:BBa_K4194013:Design

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)


p(TetR)-RBS-EGFP-LVA-RBS-pleD-LVA-terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1359
    Illegal NotI site found at 1508
    Illegal NotI site found at 1611
    Illegal NotI site found at 2258
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1597
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1811
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for Escherichia coli has been done.


Source

None

References